標題: 一些潛在的益生元和富纖維食品對狗腸道微生物的影響 [打印本頁] 作者: 牧童 時間: 2010-6-9 12:32 標題: 一些潛在的益生元和富纖維食品對狗腸道微生物的影響 Influence of some potential prebiotics and fibre-rich foodstuffs on composition and activity of canine intestinal microbiota
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Giacomo Biagi, a, , Irene Cipollinia, Monica Grandia and Giuliano Zaghinia
a Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali (DIMORFIPA), Università di Bologna, via Tolara di Sopra 50, 40064 Ozzano Emilia, Italy
Received 9 September 2009; revised 13 April 2010; accepted 15 April 2010. Available online 7 June 2010.
Abstract
The aim of the present study was to evaluate the effect on the composition and activity of the canine intestinal microbiota of different sources of soluble fibre that are among the most widely used by the pet food industry, and some prebiotic substances that might be considered as potential ingredients for dog diets. In vitro, there were 14 treatments: control diet; lactitol; Beneo P95 (FOS from partially hydrolyzed inulin from chicory); Fibrulose F97 (FOS from chicory); Fibruline XL (inulin from chicory); Pectin Classic AU202 (pectins from apple); Pectin Classic CU201 (pectins from citrus fruit); Beneo GR (inulin from chicory); gluconic acid; dried chicory; dried beet pulp; Exafine 500 (pea hull fibre); Psyllogel (850 g/kg Psyllium fibre); Benefibra (partially hydrolyzed guar gum). Substrates were added at 1 g/L (treatments 2 through 9) or 4 g/L (treatments 10 through 14) to canine faecal cultures for a 24 h incubation. In view of the different beneficial properties that they showed during the in vitro trial, lactitol and Pectin Classic CU201 were selected to be tested in a feeding study with 16 privately owned dogs. Dogs were divided into two treatment groups of 8 animals each; all dogs were fed the same commercial dry diet (the same one used for the in vitro study) and received (1) lactitol or (2) Pectin Classic CU201 at 10 g/kg of diet for 30 d. Faecal samples were collected at 0, 20, and 30 d after the start of the trial and 10 d (day 40) after prebiotic withdrawal. In vitro, compared with the control, gas production and maximum rate of gas production were increased (P<0.01) by most substrates. Pectin Classic CU201 was the only substrate that resulted (P<0.01) in a lower ammonia concentration than the control both after 6 and 24 h of fermentation. Compared with the control, lactitol, FOS, Psyllogel and Benefibra increased propionic acid and reduced the acetic + n-butyric to propionic acid ratio (P<0.01). Pectins resulted in higher acetic and n-butyric acid levels than the control (P<0.01). Compared with the control, coliforms were reduced (P<0.01) by lactitol both in vitro and in vivo (−2 log units). In vitro, several substrates resulted in higher counts of lactobacilli and enterococci than the control (P<0.01). In vivo, compared with faecal counts at trial start, lactobacilli and enterococci were not affected (P>0.05) by treatment, whereas prebiotics resulted in reduced concentrations of coliforms and Clostridium perfringens (P<0.05). This study produced evidence that different prebiotic substances and fibre-rich foodstuffs exert different effects on the composition and activity of canine intestinal microbiota.
Abbreviations: DM, dry matter; dp, degree of polymerization; FOS, fructooligosaccharides; PHGG, partially hydrolyzed guar gum; VF, maximum volume of gas produced; VFA, volatile fatty acids; λ, lag time; μm, maximum rate of gas production
Article Outline
1. Introduction
2. Materials and methods
2.1. Animal care and use
2.2. In vitro experiment
2.3. In vivo feeding trial
2.4. Chemical analysis
2.5. Bacterial counts
2.6. Statistical analysis
2.6.1. In vitro experiment
2.6.2. In vivo feeding trial
3. Results
3.1. In vitro experiment
3.2. In vivo feeding trial
4. Discussion
5. Conclusion
References作者: 李業明 時間: 2010-6-9 12:38
牧童老師可以翻譯一下不！
您那里有沒有肉兔方面的營養標準資料，如伊拉或者新西蘭等，謝謝！作者: 破殼 時間: 2010-6-9 13:29
:wenti:看。。?？?。。?？床欢?。。。作者: ppd130329 時間: 2010-6-9 23:00
望洋興嘆作者: beyond_sn21 時間: 2010-7-14 14:44
益生元在寵物食品中的應用早就很多了，只是國人對這方面的觀念還不是很重視，但真正嚴格意義上的益生元也就兩三種。關鍵是在寵物上怎么應用，選用何種益生元。作者: 2014030007 時間: 2010-7-16 13:16
暈。。。頭真的很暈作者: beyond_sn21 時間: 2012-7-12 17:47
文中的FOS貌似來源于菊粉的水解產物，這種來源的FOS缺點是短鏈含量不高，沒有來源于蔗糖的FOS利用率高。
菊粉來源的FOS作為低熱量的甜味劑使用較多